Real-time Diagnosis and Monitoring of Cytomegalovirus Infections Using TaqMan-MGB Probe Technology

TaqMan MGB Probe Fluorescence Real-Time Quantitative PCR for Rapid Detection of Chinese Sacbrood Virus

Sacbrood virus (SBV) is a picorna-like virus that affects honey bees (Apis mellifera) and results in the death of the larvae. Several procedures are available to detect Chinese SBV (CSBV) in clinical samples, but not to estimate the level of CSBV infection. The aim of this study was develop an assay for rapid detection and quantification of this virus. Primers and probes were designed that were...

Human Cytomegalovirus: Infections and Diagnosis

Human cytomegalovirus (HCMV) is a ubiquitous virus whose sole host is humans. Since HCMV can contagion from person to person through numerous ways, vast populations of humans are infected. HCMV infections can potentially have a range from asymptomatic infection in immuno-competent hosts to life-threatening diseases in organ recipients and patients with AIDS. The present article reviews the occu...

\"Internet of Things\": An Emerging Real-Time Technology for Environmental Health Monitoring

human cytomegalovirus: infections and diagnosis

human cytomegalovirus (hcmv) is a ubiquitous virus whose sole host is humans. since hcmv can contagion from person to person through numerous ways, vast populations of humans are infected. hcmv infections can potentially have a range from asymptomatic infection in immuno-competent hosts to life-threatening diseases in organ recipients and patients with aids. the present article reviews the occu...

Real-time automated PCR for early diagnosis and monitoring of cytomegalovirus infection after bone marrow transplantation.

The purpose of this study was to assess the usefulness of real-time automated PCR as a quantitative, highly reproducible, and sensitive method to detect cytomegalovirus (CMV) DNA in blood specimens. Intra- and interassay precision rates were 0.89% (small number of copies [L]), 1.43% (middle number of copies [M]), and 1.12% (high number of copies [H]), and 4.46% (L), 1.51% (M), and 2.28% (H), re...